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Fig. 3. Influence of elastin-derived peptides on cell proliferation, survival and migration. (A,B) Cell proliferation and survival: HMECs (10⁴ cells) were seeded in 24-well culture plates and cultured in FCS-free ECGM MV for various periods (A) or for five days (B) in the absence of KE (control, C) and growth factor (none) or in the presence of KE and VEGF (20 ng/ml) (b) or bFGF (20 ng/ml) (c). Cell number was determined at the end of each period of incubation. (C) To examine cell migration, HMEC monolayers were disrupted with a 1mm cell scraper and incubated in the absence or presence of KE or VGVAPG peptide and in the absence (a, none) or presence of VEGF (20 ng/ml) (b) or bFGF (20 ng/ml) (c) for 24 hours. Cell migration was determined in triplicate experiments as percentage of migrating cells relative to control value in the absence of growth factors and expressed as mean±s.d. NS, not significant; ^*P<0.05; ^**P<0.01; ^***P<0.001.

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