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Fig. 2. Live imaging of GFP-ERGIC-53 reveals two types of structures with different dynamics. (A) A whole cell and its schematic representation where paths of stationary structures imaged every 10 seconds have been traced (see supplementary material Movie 2). Note that these structures undergo very short range to no movement. Lower panel shows image series of the outlined portion in the upper panel. Arrowheads point two peripheral ERGIC structures fusing with each other. Arrows indicate elongated ERGIC structures. (B) Representative images from supplementary material Movie 3. GFP-ERGIC-53-expressing HeLa cells were imaged in the xyz directions every 10 seconds. Arrows indicate long-lasting structures that can persist for up to 31 minutes. (C) Data taken from supplementary material Movie 4 imaged with an interval of 0.2 second. Representative frames show three peripheral ERGIC structures (arrowheads) that hardly move during the entire imaging interval. The arrow points to a peripheral stationary spot shooting out an elongated structure. Lower panel shows the whole cell and a schematic representation of paths that GFP-ERGIC-53 fast-moving structures followed during the imaging time. Note that the movement does not follow a defined direction, and often seems to connect two peripheral stationary structures. Bar, 5 µm. Time is shown as minutes.seconds.
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