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Fig. 5. GFP-ERGIC-53 and VSV G-GFP take different routes from the ERGIC. Quantification of the flow rate direction of GFP-ERGIC-53 (A) and VSV-G-GFP (B) in cells rewarmed from 16°C to 37°C or 15°C to 32°C, respectively. The protein flow rate is plotted in arbitrary units (normalized to 1) against the direction of movement. Note that VSV-G-GFP is directed toward the Golgi whereas GFP-ERGIC-53 is directed away from the Golgi. These differences are statistically significant (Student's t-test, P≤0.05). Bars show the mean±s.d. (GFP-ERGIC-53, n=67; VSV-G-GFP, n=73); No, nondirectional movement.





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