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Fig. 1. Spectrin colocalizes with integrin in clusters at the periphery of newly adherent BAE cells but has a more diffuse distribution in spreading cells. BAE cells were allowed to spread on fibronectin-coated coverslips for 30 minutes (A), 1 hour or 8 hours (B), fixed, permeabilized and dual-labeled with monoclonal antibody against {alpha}vß3 integrin and polyclonal antibodies against spectrin. (A) Three independent fields of cells are shown and cells containing integrin clusters are indicated with arrows. (B) Focal adhesions are indicated with arrows. Bar, 20 µm.





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