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Fig. 8. SH3 domain of spectrin accumulates in the early integrin clusters. BAE cells were transiently transfected with plasmid encoding HA epitope-tagged SH3 domain. Cells were detached and replated on fibronectin for 1 hour, fixed and permeabilized. Cells were stained with monoclonal antibody against HA epitope and polyclonal antibodies against an autolytic fragment of calpain. (B) Confocal analysis was performed on horizontal (x-y plane) optical sections (0.3 µm intervals); three sections going from the upper to the lower region (left to right) of a single cell are shown. Arrows indicate calpain-fragment- and SH3-domain-containing clusters. Bar, 20 µm (A); 5 µm (B).
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