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Fig. 3. Solubility properties of lamins and lamina-associated proteins during myogenesis. C2C12 myoblasts were induced to differentiate with 2% horse serum. At 0, 72 and 120 hours after transfer to differentiation medium, cells were harvested and subjected to nuclear isolation. Nuclei were either solubilized in SDS (nuclei) or sequentially extracted with hypotonic (LS) or hypertonic (HS) buffer. Samples were resolved by SDS-PAGE along with material resistant to extraction (INS), transferred to nitrocellulose and blotted with antibodies against lamin B1 (a), lamin B2 (b), lamin A (c) and lamin C (d), and the intensity of each band evaluated by densitometry and expressed as a proportion of each protein in whole nuclear extracts.





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