JCS01622 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Effect of Ror1 and Ror2 suppression by RNAi on stage II hippocampal neurons. Hippocampal cultures were transfected with Ror1 or Ror2 siRNA duplexes 2 hours after plating. As controls, cultures were transfected with scrambled duplexes or were left untreated. After 1 day in culture, control (A,D,G,J), scrambled siRNA Ror1-treated (B,E), scrambled siRNA Ror2-treated (H,K), siRNA Ror1-treated (C,F) and siRNA Ror2-treated (I,L) hippocampal neurons were double stained with tubulin (A-C, G-I) and Ror1 (D-F) or Ror2 (J-L) antibodies. Ror protein loss in the targeted neurons was accompanied by the elongation of shorter and less branched minor processes (mp) than seen in the control (arrows in F,L). Bar, 20 mm.

• Supplemental Figure 2 -

  Fig. S2. Effect of Ror1 and Ror2 suppression by RNAi on stage III hippocampal neurons. Hippocampal cultures were transfected with Ror1 or Ror2 siRNA duplexes 2 hours after plating. As controls, cultures were transfected with scrambled duplexes or were left untreated. After 2 days in culture, control (A,D,G,J), scrambled siRNA Ror1-treated (B,E), scrambled siRNA Ror2-treated (H,K), siRNA Ror1-treated (C,F) and siRNA Ror2-treated (I,L) hippocampal neurons were double stained with tubulin (A-C, G-I) and Ror1 (D-F) or Ror2 (J-L) antibodies. Ror-depleted neurons elongated shorter minor processes (mp) but longer axons (ax) compared to those observed in control cells (arrows in F,L). Bar, 20 mm.