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Fig. 2. Mcp6 is a meiosis-specific SPB-associated protein. (A) Microscopic analysis of Mcp6 localization during meiosis. The mcp6+-gfp dsred-sad1+ strain (ST142) was induced to enter meiosis by nitrogen starvation. After 6 hours of incubation, the cells were collected and fixed with methanol for microscopic observation. The GFP signal is green, the DsRed signal is red and Hoechst 33342 staining is blue. (B) Mcp6-GFP localizes to the SPB but not to the telomeres in mitotic cells. Overproduction by transforming mitotic cells with the pRGT81 (GFP expression vector) or mcp6+/pRGT81 (Mcp6-GFP expression vector) plasmid is indicated by `OP'.





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