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Fig. 4. Homologous pairing is reduced in mcp6{Delta} cells. (A) Time-lapse observation of the lys1 locus in a living cell, either mcp6{Delta} (ST197) or wild type (WT) (AY174-7B), during the horsetail stage. The lacO repeat sequence integrated into the lys1 loci was visualized by the LacI-NLS-GFP fusion protein. Images of a single cell were obtained at 5 minute intervals. The numbers at the bottom of each photograph represent the timing in minutes, with 0 minutes being when nuclear fusion (karyogamy) occurs. The rectangles under each photo indicate that the lys1 loci were paired (grey) or not paired (white). Bar, 5 µm. (B) Time course of homologous pairing frequency during the horsetail stage in mcp6{Delta} (red square) compared with that in the WT (blue circle). The average values were calculated from 20 independent cells. The lys1 locus in chromosome I is illustrated as an inset.





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