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First published online October 11, 2005

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Flagella make waves

Flagella are supported by a scaffold termed the axoneme, which comprises nine pairs of microtubules surrounding a central apparatus of two singlet microtubules (C1 and C2) joined by projections. The complex waveforms beating flagella produce arise through spatial regulation of the motor protein dynein along the axoneme. In Chlamydomonas, the central apparatus modulates flagellar waveforms, but its composition is largely undefined. Now, on p. 4655, Elizabeth Smith and co-workers identify calmodulin, PF6 and four novel proteins as components of a complex that localizes to the C1 microtubule. The pf6 mutant of Chlamydomonas, which lacks the C1a projection, has flagella that only twitch. The authors use anti-PF6 antibodies to immunoprecipitate five additional components of the C1a projection, which they then identify by mass spectrometry. They show that all five proteins plus PF6 form a single complex and identify specific binding partners within the complex. Although four of the polypeptides are novel proteins of unknown function, the fifth polypeptide is calmodulin. This result strongly implicates the axonemal central apparatus in modulation of flagellar waveforms by calcium, an important regulatory mechanism.

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Related articles in JCS:

Calmodulin and PF6 are components of a complex that localizes to the C1 microtubule of the flagellar central apparatus

Matthew J. Wargo, Erin E. Dymek, and Elizabeth F. Smith
JCS 2005 118: 4655-4665. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?