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Fig. 2. Male sporogenesis and meiosis of wild-type (A,B,E-I) and the Atscc3-1 mutant (C,D,J-S). (A,D) DIC microscopy of male meiocytes (A,C) and of the products of male meiosis (tetrads of microspores, B and D). (E-S) DAPI staining of wild-type and mutant pollen mother cells during meiosis. (E) Pachytene, (F) diakinesis, (G) metaphase I, (H) anaphase I, and (I) end of anaphase II in wild-type meiocytes. The same stages are shown in Atscc3-1 mutant pollen mother cells: (J,O) pachytene, (K) diakinesis, (P,L,Q) metaphase I, (M,R) anaphase I and (N,S) anaphase II. Asterisks indicate pericentromeric heterochromatin during pachytene stages (E,J,O). Arrows indicate some of the abnormalities observed in the Atscc3-1 mutant: synapsis defects at pachytene (J,O), chromosome bridges (M) and chromosome fragmentation (R). Bar, 10 µm.





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