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Fig. 1. (A,B) Western blots of axonemes and silver-stained gel of immunoprecipitates. (A) Western blot of axonemes isolated from wild-type (wt) and pf6-2 Chlamydomonas reinhardtii cells probed with our anti-PF6 antibodies. A band with a molecular weight consistent with that of PF6 is present in wild-type axonemes and absent in pf6-2 axonemes. (B) Silver-stained gel of immunoprecipitates obtained from wild-type (wt) and pf6-2 axonemal extracts using our anti-PF6 antibodies. In wild-type axonemal extracts, five polypeptides in addition to PF6 are specifically precipitated using anti-PF6 antibodies. These polypeptides are not precipitated in pf6-2 axonemal extracts. The precipitating immunoglobulin heavy and light chains are labeled HC and LC, respectively.





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