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Fig. 3. Western blots of axonemes isolated from wild-type (wt) and pf6-2 cells probed with antibodies generated against PF6-IP1, -IP2, -IP3, -IP4 and calmodulin (CaM). (A) Blots of 4-16% gradient polyacrylamide gels. (B) Blots of 15% polyacrylamide gels. The anti-PF6-IP4 antibody recognizes a polypeptide of the correct size (asterisk). With the exception of calmodulin, each of the proteins is missing from pf6-2 axonemes.





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