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Fig. 7. (A,B) Silver-stained gels and corresponding western blots of immunoprecipitates. (A) Silver-stained gel of immunoprecipitation experiments using undialyzed, high-salt axonemal extracts obtained from wild-type flagella. The precipitating antibody is indicated at the top of each lane. The immunoglobulin heavy and light chains are labeled HC and LC, respectively. (B) Corresponding western blots from immunoprecipitation experiments. The blots were probed with the antibodies indicated to the right of each panel. In high-salt conditions, PF6-IP1 and calmodulin co-precipitate, and PF6-IP2, -IP3 and -IP4 co-precipitate. Only very minimal amounts of complex components precipitate with PF6.





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