spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 9. Western blot of gel overlay assay. Axonemes from wild-type (wt) and mutant axonemes were subjected to SDS-PAGE and transferred to nitrocellulose membrane. The blot was then incubated with bacterially expressed PF6-IP3. The bound PF6-IP3 was detected using S-protein overlay (see Materials and Methods). PF6-IP3 binds to a polypeptide of molecular weight consistent with that of PF6-IP2 (asterisk). This polypeptide is absent in axonemes isolated from pf6, pf16 and pf18, also consistent with PF6-IP2.





Right arrow Return to article