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Fig. 3. Bves localization at the TJ in vivo. (A) Cross sections through adult mouse small intestine were labeled with antibodies to Bves and occludin (top row), ZO-1 (middle row), or E-cadherin (bottom row). Merged images show a high degree of overlap of Bves with TJ proteins at an apicolateral position. Scale bar: 10 µm. (B) Ultrathin sections were labeled with Bves and detected using a 5 nm colloidal gold-conjugated antibody. Quantitative analysis of immunogold location shows the percentage of total beads that lie within a specific distance from the apical surface. The greatest number of beads is found in the domain where the TJ is located (red bar). (C) Cross sections of gastric epithelium show junctional components TJ, AJ and desmosomes vertically oriented along the lateral membrane (a,b,c). As seen in higher magnification, colloidal gold beads are clustered within 300 nm of the cell surface in the TJ domain (white arrows, a',b',c'). Occasionally, beads are detected below the TJ (c, green arrow) in regions where AJs and desmosomes are typically located. En face sections show tangentially sectioned microvilli with colloidal gold beads easily visible in a region within 300 nm from the cell membrane (d',d''). Scale bars: 100 nm.
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