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Fig. 6. Bves incorporation into TPA-induced membrane structures. MDCK cells were cultured in serum-free medium, switched to EGTA-containing medium, and either returned to serum-free medium (A,C,E) or treated with TPA (B,D,F). Following TPA treatment, Bves, occludin and ZO-1 are found in TPA-induced TJ-like structures at the cell membrane (B,D, arrows). E-cadherin labeling becomes diffuse and does not localize to these structures (F, arrows). Scale bar: 10 µm.