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Fig. 1. Irrelevant cytoplasmic tails on DRß overcome the Iip35 ER retention motif. (A) Schematic representation of the chimeric class II and ß chain combinations expressed in HEK 293T cells. (B) HEK 293T cells were transiently co-transfected with cDNAs for the indicated chimeric molecules and Iip35 LI/ML. After 48 hours, cells were analysed for Ii expression by flow cytometry using BU45 either before (surface) or after (total) permeabilization with saponin. (C) The ratios of surface to total Ii are shown next to the C-terminal amino-acid sequences of the chimeric molecules' cytoplasmic tails. Class-II expression was measured on permeabilized cells using the ISCR3 mAb. The mean fluorescence values (MFVs) for DR TM/ß, DR TM/ß, DR TM/ßcytoDMmut and DR TM/ßcytoDO expression were 280, 267, 275 and 251, respectively. The MFV for untransfected cells was 4 (data not shown).

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