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Fig. 6. Proline residues in the Iip35 cytoplasmic tail are not required for function of the RXR motif and its masking by HLA-DR. (A) N-Terminal amino acid sequence of the Iip35 proline mutants. Positions 1 and 17 are the initiation sites of Iip35 and Iip33, respectively. The arginine residues in the RXR motif are underlined, the leucine-based targeting signals are in black boxes, prolines in Iip33 region are in bold, and Ser6 and Ser8 are boxed. (B) HEK 293T cells were transiently co-transfected with the indicated invariant-chain molecules and with or without DR 
TM/ß and tested after 48 hours. Cells were analysed by flow cytometry using BU45 either before (surface Ii) or after (total Ii) permeabilization with saponin. The ratios of surface to total Ii are shown. (C) Schematic representation of the p33 cytoplasmic tail structure as determined by nuclear magnetic resonance (Motta et al., 1997). Proline residues that were mutated to alanine are indicated.
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