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Fig. 2. Long-term time course of Ca²⁺-triggered granule fusion. Granule light scattering, a semi-quantitative measure of granule fusion, was measured at intervals after the addition of Ca²⁺ buffer to give the following final concentrations: 0.001 µM (IM buffer), ; 11.9 µM, ; 572 µM, ; 1151 µM, . The decrease in light scatter characteristic of fusion in this system was complete at the two higher Ca²⁺ concentrations at the earliest interval (3 minutes after Ca²⁺ buffer addition) measurable. No further change in light scattering was recorded over the remaining measurement interval. Additional Ca²⁺ buffer was added at the 65-minute time point, resulting in the following approximate final concentration levels: <0.001 µM (IM buffer), ; 572 µM, ; 1450 µM, ; 2680 µM, . The granules were pelleted by centrifugation at the 110-minute time point, concentrating them in the well bottom. No change in absorbance, indicative of further fusion, was observed in either case. Error bars indicate s.d. (n=3).

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