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Fig. 5. Restoration of tethering by factors present in KI salt wash. (A) KI-washed vesicles (tether incompetent) received IM buffer only or (B) IM buffer conditioned by the addition of protein factors (40 µg desalted into IM buffer) stripped from granules during incubation in KI. Tethering is restored by these factors. (C) Fusion products produced by Ca2+ addition to KI-washed granules. (D) Fusion products produced by Ca2+ addition (300 µM) to granules tethered, as in B by the prior addition of tethering factors (TF) from a KI wash of granules. Note the increased size of the resultant fusion products. Bar, 10 µm.





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