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Fig. 2. Alignment of human dermal fibroblasts in a collagen matrix subjected to radial interstitial flow. Confocal images of cells (A) and matrix fibers (B) with their corresponding FFT analyzed intensity frequency histograms. f-actin is labelled green with the confocal reflection in red; arrow indicates flow direction. These observations were quantified by alignment index (C) and peak angle (D) for cell and matrix alignment, respectively. Unpaired t-tests were used for statistical analysis of the means; significant differences (**P<0.01) were observed in alignment index in both cells and matrix under flow conditions compared to that measured under static conditions using Mann-Whitney test. Bar, 200 µm (A); 20 µm (B).
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