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Fig. 5. The transport of the prApe1 to the vacuole is blocked in the {Delta}mon2 mutant. (A) YFP95 (wild type) and YFP93 ({Delta}mon2) were metabolically (35S) labeled at 30°C for 10 minutes and chased for the indicated times. The ER, Golgi and mature forms of CPY are labeled p1, p2 and m, respectively. (B) SEY6210 (wild type) and AAY1120 ({Delta}mon2) cells were metabolically (35S) labeled at 26°C for 20 minutes and chased for the indicated times. CPY (A) and Ape1 (B) were immunoprecipitated from extracts and analyzed by SDS-PAGE and autoradiography. (C) Subcellular localization of GFP-prApe1. SEY6210 and AAY1120 cells expressing GFP-prApe1 were grown at 26°C to mid-logarithmic phase and labelled with FM4-64 to visualize vacuoles. Analysis was by fluorescence microscopy and images shown are representative of more than 100 cells observed.





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