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Fig. 6. EM analysis of subcellular structure. SEY6210 (wild-type) (A), AAY1120 (
mon2) cells grown at 26°C (B,C,D) and shifted 38°C for 2 hours (F,H), and AAY104 (pik1ts) cells pre-shifted to the restrictive temperature for one hour (E,G) were processed for and visualized by EM as described in Materials and Methods. Black arrowheads indicate enlarged aberrant membrane structures shown enlarged in the panels on the right. Vacuoles (v), nuclei (n) and mitochondria (m) are also indicated. Cells shown are representative of >90% of the population (except for multinucleation, which was observed in 
15% of Dmon2 cells). Bar, 0.5 µm.
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