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Fig. 8. Isolation of ARL1, NEO1 and DOP1 as multicopy suppressors of the ${Delta}$ mon2 mutant. ARL1, NEO1 and DOP1 were isolated in CDK13-1A ( ${Delta}$ mon2/ ${Delta}$ tif3) double mutant as described in Materials and Methods. The complementation of the ${Delta}$ mon2 mutant was subsequently tested in YFP44 ( ${Delta}$ mon2/chc1-521^t^s) double mutant. YFP44 cells were transformed with plasmids (2 µ, and the appropriate auxotrophic markers) alone or plasmids containing MON2 (pFP45), DOP1 (pDK64), NEO1 (pRB111) or ARL1 (pRB95). The resulting transformants were then tested for growth at 30°C on 5-FOA-containing plates as indicated. No growth complementation was observed in YFP86 ( ${Delta}$ tif3/vps54) double mutant (data not shown), which was used as a control.

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