Actin and non-muscle myosin II facilitate apical exocytosis of tear proteins in rabbit lacrimal acinar epithelial cells
J Cell Sci Jerdeva et al.
118: 4797
JCS02573 Supplementary Material
Files in this Data Supplement:
Movie 1
-
Movie 1.Time-lapse confocal fluorescence microscopy of the
resting acinus shown in Fig. 3 co-transduced to express GFP-actin.Images were acquired at 10.5-second
intervals over 16 minutes.
Movie 2
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Movie 2.Time-lapse confocal fluorescence
microscopy of the acinus shown in Fig. 4A co-transduced to express GFP-actin
and then exposed to CCH (100 mM)
at the onset of image acquisition.Images were acquired at 10.5-second intervals over 16 minutes.
Movie 3
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Movie 3.
Time-lapse confocal fluorescence microscopy of the acinus shown in Fig. 4B
co-transduced to express GFP-actin and exposed to CCH (100 mM) at the onset of image acquisition.Images were acquired at 5-second
intervals over 17 minutes.
Movie 4
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Movie 4.Time-lapse confocal fluorescence
microscopy of the acinus shown in Fig. 7A co-transduced to express GFP-actin,
exposed to BDM (10 mM, 15 minutes), and exposed to CCH (100 mM) at the onset of image
acquisition.Images were acquired
at 11-second intervals over 17 minutes.
Movie 5
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Movie 5.Time-lapse confocal fluorescence
microscopy of the acinus shown in Fig. 7B co-transduced to express GFP-actin,
exposed to ML-7 (40 mM,
15 minutes), and exposed to CCH (100 mM)
at the onset of image acquisition.Images were acquired at 5-second intervals over 15 minutes.
