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Fig. 10. EM analysis of SV diameter in lacrimal acini exposed to BDM or ML-7, then CCH. The maximal SV diameter of vesicles categorized as single, dual fused or multiple fused was measured as described in Materials and methods in untreated lacrimal acini (CON) or acini treated without or with BDM (10 mM, 15 minutes) or ML-7 (40 µM, 15 minutes) prior to CCH (100 µM, 5 minutes). Vesicle diameter per category under each experimental condition is shown in the histogram while a sample of the vesicle type is outlined in blue in the EM image to the right. Arrows, filaments associated with a SV cluster; bars, 1 µm.
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