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Fig. 6. BDM and ML-7 alter CCH-stimulated actin remodeling while inhibiting protein secretion in lacrimal acini. (A) Confocal fluorescence micrographs of control (CON), BDM-pretreated (10 mM, 15 minutes) or ML-7-pretreated (40 µM, 15 minutes) lacrimal acini exposed to 100 µM CCH for 5 minutes, and then fixed and processed to detect myosin II (green, also shown as monochrome image) and actin filaments (red, also shown as monochrome image). Arrows, recruitment of myosin II immunoreactivity to areas enriched in actin-coated fusion intermediates; *, lumena; bar, 5 µm. (B) Bulk protein secretion in acini treated with BDM and ML-7 as described above and stimulated with CCH for 5, 10 or 30 minutes. Basal release, total release and the stimulated component (total minus basal) are each plotted. Values were normalized to cell protein before comparison across samples. Bars, s.e.m.; *significant at P≤0.05 from comparable value in control cells and n=5-6 preparations.





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