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Fig. 7. Time-lapse confocal fluorescence microscopy of GFP-actin in lacrimal acini exposed to BDM or ML-7, then CCH reveals stabilization of actin-coated structures. Lacrimal acini transduced to express GFP-actin and exposed to BDM (10 mM, 15 minutes, A) or ML-7 (40 µM, 15 minutes, B) were imaged immediately upon CCH addition (100 µM). Selected images of GFP-actin fluorescence at intervals throughout the time-lapse sequence are shown. Arrowheads, actin-coated structures; arrow, actin bubble at the basolateral membrane; bar, 5 µm.





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