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Fig. 8. BDM and ML-7 reduce CCH-stimulated exocytosis of syncollin-GFP in lacrimal acini. (A) Confocal fluorescence microscopy images acquired at the indicated times after CCH addition (100 µM) without inhibitor treatments (CCH) or with ML-7 treatment (40 µM, 15 minutes) or BDM treatment (10 mM, 15 minutes) prior to CCH addition. DIC and overlay images are also shown at 0 seconds. *, Lumen; bars, 5 µm. Plots to the right of each treatment group depict 2.5 D graphical reconstructions of the overall intensity profile of the imaged areas at 0 and 600 seconds of stimulation with CCH, illustrating individual intensities per pixel utilizing the rainbow scale. The resolution is 
10 pixels per µm. (B) Syncollin-GFP release (plotted as percentage of control) in resting and CCH-stimulated (100 µM, 30 minutes) acini without or with BDM and ML-7 pretreatment as described above. *Significant from paired control at P
0.05. Although the time-lapse sequences indicate rapid release of syncollin-GFP, the signal in the culture medium reached appropriate intensity for analysis by western blotting after 30 minutes, likely due to the time taken for exocytosed material in the lumen to diffuse into culture medium.
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