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Fig. 5. MyoD expression rises with age. (A) In situ mRNA hybridization shows myoD mRNA is lower in 2 month (inset shows a rare myoD+ fibre region) than 24 month superficial TA fibres. Bar, 100 µm. (B) Both MyoD protein and mRNA increase in myonuclei with fibre maturation. (C) X-gal reaction of wholemount TA and EDL muscle from MD6.0-lacZ myoD reporter mice is stronger at 24 months than 2 months. (D) Quantified ß-gal activity in TA muscle homogenates increases between 2 months and 12 months of age. *P<0.05, **P<0.01, ***P<0.001.