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Fig. 1. MYO1B co-distributes with actin filaments at the cell periphery and partially with endosomes in MNT1 cells. (A-C) MNT-1 cells were co-labelled with affinity-purified polyclonal anti-Myo1b antibodies (SE5237) and phalloidin and analysed using confocal microscopy. Same optical section throughout the focal plane of the nucleus of one MNT-1 cell is shown for (A) Myo1b and (B) actin. Overlay of A and B is shown in C. Arrows show membrane areas enriched in actin filaments. Bar, 2.2 µm. (D-F) MNT-1 cells were co-labelled with affinity-purified polyclonal anti-Myo1b antibody (SE5237) and anti-EEA1 antibody. Same optical section throughout the focal plane of the nucleus of one MNT-1 cell for (D) Myo1b and (E) EEA1. An overlay of D and E and is shown in F. Arrows imdicate the partial co-distribution between endosomes and Myo1b. Bar, 1.9 µm.