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Fig. 8. EGFP-Myo1b and MYO1B co-immunoprecipitate with the precursor P2 of Pmel17. (A) Schematic representation of recombinant EGFP-Myo1b and Myo1b-truncated domains. Myo1b has a motor domain with ATP-and actin-binding sites at the N-terminus and a tail with IQ motifs and a cluster of basic amino acids at the C-terminus. The motor domain was deleted in EGFP-Myo1b-Tail. (B) EGFP or EGFP-Myo1b cell lysates were immunoprecipitated with anti-GFP antibodies and analysed by SDS-PAGE, and western blotting with anti-GFP antibody and the anti-Pmel17 antibody {alpha}Pep13. (C) EGFP, EGFP-Myo1b and EGFP-Myo1b-Tail cell lysates were immunoprecipitated with the anti-Pmel17 antibody (HMB50) and analysed by SDS-PAGE, and western blotting with anti-GFP antibody. EGFP and EGFP-Myo1b cell lysates immunoprecipitated with HMB50 antibody were also analysed by SDS-PAGE, and western blotting with anti-Myo1b antibody (SE7995). These experiments were repeated three and four times for B and C, respectively.





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