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Fig. 2. Effects of mßcd on CV docking. (A) Treatment with 0.5 mM mßcd does not alter CV density at the cortex, but affects fusion of CVs after perfusion of 113±15 µM [Ca2+]free; overlay shows unfused vesicles in white, fused vesicles in green. (B) Lysis of CV-PM preparations and (C) suspensions of isolated CVs after treatment with the indicated concentrations of mßcd (n=3).





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