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Fig. 5. Effects of cyclodextrins on CV-CV fusion. CVs treated with 0 mM (closed circles) or with (A) concentrations of {alpha}cd (n=2), or ßcd as indicated (n=2). Dashed lines in A represent the 99% confidence intervals of the fusion plot for untreated CVs. (C) Total CV cholesterol assayed after treatment with the indicated cyclodextrins. Results are expressed as percentage of untreated control CVs. *, **significant difference from control and all other conditions (P<0.001). (D) HPTLC analysis of supernatant extracts following incubation with 0 mM or 2 mM of the indicated cylcodextrin. Lipid standards include cholesteryl esters (CE), triacylglycerol (TAG), free fatty acids (FFA), diacylglycerol (DAG), cholesterol (Chol), monoacylglycerol (MAG), ceramide (Cer), cardiolipin (Card), diacylphosphatidylethanolamine (PE), diacylphosphatidylinositol (PI), lysophosphatidylethanolamine (LPE), diacylphosphatidylserine (PS), diacylphosphatidylcholine (PC), lysophosphatidylserine (LPS), sphingomyelin (Sph), and lysophosphatidylcholine (LPC). The prominent band in the mßcd lane is mßcd. (E) Kinetics of CV-CV fusion following treatments as in A, in response to 87±3 µM [Ca2+]free (n=2).





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