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Fig. 7. The effects of supplementing native, untreated CVs with cholesterol using hpßcd. (A) Ca2+ activity curves of CVs treated with 0 mM or 5 mM hpßcd-cholesterol (n=3). Inset: total CV cholesterol of samples: 1, untreated CVs; 2, treated with 5 mM hpßcd-cholesterol. Results are presented as percentage of control cholesterol; *significant differences from control (P<0.001). (B) Kinetics of CV-CV fusion in response to 96±11 µM [Ca2+]free (n=3) as in A.





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