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Fig. 4. TGF-ß1-induced EMT in the NMuMG cell line regulates localization and expression of paxillin and Hic-5. (A) Hoffman modulation contrast and indirect immunofluorescence microscopy of NMuMG cell line either unstimulated (a,c,e) or stimulated with 2 ng/ml TGF-ß1 for 48 hours (b,d,e). Hoffman images (a,b), paxillin (clone 165 paxillin-specific monoclonal antibody) (c,d) and the actin cytoskeleton visualized using Rhodamine-conjugated phalloidin (e,f). Induction of an EMT leads to the relocalization of paxillin from a mainly cytoplasmic distribution to a predominant focal adhesion localization at the ends of actin stress fibers. (B) Induction of Hic-5 expression and focal adhesion localization following an EMT. Indirect immunofluorescence microscopy of NMuMG cells either unstimulated (a,c) or stimulated with 2 ng/ml TGF-ß1 for 48 hours (b,d). Hic-5 (a,b), actin cytoskeleton (c,d). Scale bar, 10 µm.





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