spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 2. Inhibition of PIP3-dependent Akt activation by the various PH domains. COS-7 cells were transfected with a HA-tagged full-length Akt construct with or without a construct encoding a plasma-membrane-targeted PI 3-kinase {gamma} (PI3K{gamma}-CAAX) and the indicated PH-GFP constructs. One day after transfection and after 6-8 hours of serum deprivation, cells were lysed and the activity of HA-Akt was measured after immunoprecipitation as detailed under Materials and Methods. Equal expression of HA-Akt, as well as the other expressed proteins, was ensured by keeping the total transfected DNA equal by complementing with the respective empty plasmid DNAs during transfection and were determined from the total cell lysates. In each experiment, the Akt activities were normalized to the level observed with PI3K{gamma}-CAAX in the presence of GFP alone, which gave an average of tenfold increase over the basal. Mean±s.e.m. of three similar observations are shown.





Right arrow Return to article