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Fig. 6. The dymanics of expression of various PH-GFP chimeras in COS-7 cells. COS-7 cells were transfected with the indicated PH-GFP constructs and the GFP fluorescence was monitored as a function of time using a fluorescence plate reader. Fluorescence values were normalized to the first reading in each group (20 hours after transfection) as shown for a representative experiment in (A). To determine the PIP3-dependent component of the effects of the expressed PH domains on the balance of proliferation/apoptosis, the ratios of the fluorescence of cells expressing the wild-type PH domains were calculated using the mutant forms of the same domain, which are incapable of lipid binding, as a control at each time point for each PH domain (B). Mean±s.e.m., n=3.
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