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Fig. 3. Silencing Cenp-F delays mitotic exit. HeLa cells expressing a GFP-tagged histone H2B fusion protein were transfected with siRNA duplexes then analysed 48 hours later by time-lapse microscopy. (A) Images from time-lapse sequences showing normal chromosome alignment and segregation in a control cell, and aberrant mitoses in Cenp-F RNAi cultures. Numbers indicate time in minutes. (B) Graph showing the time in minutes from early prometaphase to mitotic exit showing that Cenp-F repression delays mitotic exit. Ten cells were analysed per group. Note that Cenp-F-deficient cells were identified by their aberrant chromosome morphologies, hence the time-lapse sequences start after prophase.





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