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Fig. 6. Merotelic attachments form in the absence of Cenp-F. (A) Control metaphase stained to detect Bub1 (green) and tubulin (red), and enlargements showing examples of bioriented kinetochore pairs with K-fibres terminating at kinetochores. (B,C) Cenp-F-deficient cells stained to detect DNA (red), tubulin (green and red), Bub1 (green) and ACA (blue), as indicated. The enlargements show (a) a merotelic oriented kinetochore pair; (b,c) mono-oriented kinetochores lacking sisters; and (d) a merotelic oriented kinetochore that lacks a sister. Bar, 5 µm when the entire spindle is shown, and 1 µm in the enlargements.