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Fig. 3. Triton-soluble and -insoluble protein fractions were analysed regarding their amounts of E-cadherin, 
- and ß-catenin in PANC-1 and BxPC-3 cells after treatment with TGFß1 (10 ng/ml) or solvent for the time points indicated (A). Equal amounts of each fraction were separated by SDS-PAGE and blotted onto nitrocellulose. E-cadherin, - and ß-catenin were detected by immunostaining. The ß-actin concentration served as control to prove equal loading. Representative blots out of four independent experiments are shown. (B) E-cadherin was precipitated from lysate of TGFß1-stimulated or unstimulated PANC-1 cells and coprecipitated - and ß-catenin was analysed by western blotting. After serum starvation for 24 hours, cells were incubated for 90 minutes either with 100 µM PP1 to inhibit Src-kinase, 25 µM PD98059 to inhibit MEK-1 or with 25 µM LY294002 to inhibit PI3-kinase and stimulated with 10 ng/ml TGFß1 or solvent for additional 6 hours. E-cadherin was precipitated from 1 mg of NOP lysate. The amount of co-immunoprecipitated - and ß-catenin was examined by immunoblotting. The blots were restained for E-cadherin to document equal amounts of precipitated protein. Three independent experiments were performed.
