spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 6. (A) Immunoprecipitation of E-cadherin, {alpha}- or ß-catenin was performed from 500 µg of PANC-1 RIPA lysate and PTEN was detected by immunoblotting. Equal amounts of immunoprecipitated proteins were documented by restaining the blots with the appropriated antibody. (B) Beta-catenin was precipitated from 2 mg of BxPC-3 or PANC-1 lysates treated with 10 ng/ml TGFß1 or solvent. Co-precipitated PTEN was detected by western blotting. The blots were restained with anti-ß-catenin antibody to demonstrate equal amounts of protein. (C) E-cadherin was precipitated from 0.5 mg of total lysate from PANC-1 cells transiently transfected with PTEN, PTEN{Delta}C or vector alone (mock). Co-precipitated {alpha}- and ß-catenin was determined by western blotting. Restaining of the blot for E-cadherin confirmed equal amounts of precipitated proteins. In all experiments representative blots out of three independent studies are shown.





Right arrow Return to article