spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 7. Treatment with effective compounds does not affect solubility of PrPc when transport of PrP along the secretory pathway is inhibited. 10 µg/ml Brefeldin A (BFA) were added to the culture medium of wtN2a cells. After 2 hours, cells were exposed to 200 µg/ml suramin, NF449 or NF023 in the presence of BFA and incubated at 37°C overnight. Cells were then harvested and subjected to a solubility assay. Soluble (S) and insoluble (P) fractions were analysed by immunoblotting, using the monoclonal anti-PrP antibody 4H11. Positions of molecular size markers are depicted on the left. Treatment of cells with BFA alone did not affect the solubility of PrPc (lane 3). Treatment with BFA in the presence of suramin, NF449 or NF023 significantly decreased the induction of PrPc aggregates (lanes 7,11 and 15).





Right arrow Return to article