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Fig. 2. Internalized MT1-MMP colocalizes with endosomal and centrosomal markers in MCF-MT-WT cells. (A) Following a 30-minute and a 45-minute uptake of the MT1-MMP antibody Ab815, the cells were stained for Rab-4 and Rab-11, respectively (both in red) and for Ab815 (green). Boxed areas are enlarged in the small panels. (B) Following the MT1-MMP antibody Ab815 uptake for 45 minutes, the cells were stained for Ab815 (green) and {gamma}-tubulin (red). Where indicated, cells were pre-treated for 1 hour with nocodazole (1 µg/ml) or Brefeldin A (5 µg/ml). Arrows indicate the centrosomes. DAPI nuclear staining is blue.





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