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Fig. 6. Antibody uptake in MCF-MT- ${Delta}$ CT cells and delivery of the soluble MT1-MMP constructs. (A) MCF-MT- ${Delta}$ CT cells were treated with the MT1-MMP antibody Ab815. The antibody was then delivered to the cells by Chariot delivery (upper panel) and by antibody uptake (lower panel). The cells were stained for ${gamma}$ -tubulin (red) and Ab815 (green) 2 hours after Chariot delivery and 45 minutes after antibody uptake. Notice that the mutant was transported to the plasma membrane but it was not efficiently internalized and transported to the centrosomes. (B) MCF-zeo cells were treated with the PEX- ${Delta}$ TM/ ${Delta}$ CT and PEX- ${Delta}$ TM constructs. The constructs were then delivered to the cells by Chariot delivery. Two hours after delivery, the cells were stained for MT1-MMP (green), golgin-97 and ${gamma}$ -tubulin (both in red). Notice that the delivered constructs do not colocalize with ${gamma}$ -tubulin and golgin-97, and appear to accumulate in the trans-Golgi network. Arrows indicate centrosomes. DAPI nuclear staining is blue.

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