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Fig. 6. Analysis of synapsis and pairing in set1 ${Delta}$ , clb5 ${Delta}$ and set1 ${Delta}$ clb5 ${Delta}$ cells. (A) Frequencies of nuclei with paired cosmid signals during meiotic time courses; wild-type and set1 ${Delta}$ strains (left graphs), or the wild-type, clb5 ${Delta}$ and set1 ${Delta}$ clb5 ${Delta}$ strains (right graphs). Top graphs: frequencies of nuclei displaying only a single signal due to pairing of a cosmid probe corresponding to a telomeric region of the chromosome III left arm (cos m; 37). Bottom graphs: frequencies of nuclei displaying paired signals with a cosmid probe hybridizing an internal region of the chromosome XI left arm (cos f; 37). (B) Representative images of Zip1-positive nuclei at 330 minutes (top) or 270 minutes (bottom) after induction of meiosis. WT, wild-type nuclei displaying thread-like Zip1 signals (green); set1 ${Delta}$ nuclei displaying Zip1 speckles only. In set1 ${Delta}$ clb5 ${Delta}$ this is only the case of a few nuclei in the culture, whereas in clb5 ${Delta}$ a few nuclei display a Zip1 polycomplex (bright green oblong). Bar, 5 µm.

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