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Fig. 8. Localization of Ndj1 and Rap1 is normal in set1 ${Delta}$ meiotic nuclei. (A) Frequency of nuclei from wild-type and set1 ${Delta}$ diploid (HA)-tagged Ndj1 strains displaying Ndj1-HA signal at the indicated time point in sporulation medium. (B) Colocalization of Ndj1-HA immunofluorescence signals (red) and XY' telo-FISH signals (green) in representative wild-type and set1 ${Delta}$ nuclei. Bar, 5 µm. (C) Immunolocalization of Rap1 (middle panels) and Ndj1-HA (right panels) in mildly spread wild-type (top panels) and set1 ${Delta}$ (bottom panels) nuclei. Single immunofluorescence channels are shown in gray scale for better sensitivity. Left panels: merged color images of immunofluorescence (green: Rap1; red: Ndj1-HA). A Ndj1-HA-positive spot with no detectable Rap1 signal is indicated (white arrowhead). Bar, 5 µm.

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