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Fig. 1. Partial karyotype and CBP expression of late passage HMEC-E6 cells (passage 20). (A) Partial karyotype of a single mitotic cell demonstrating two copies of an unbalanced translocation between chromosomes 13 and 16 involving 16p13 (arrows). Spectral karyotyping in classification colors (red, chromosome 13 material; orange, chromosome 16 material). (B) Partial karyotypes of two cells that demonstrate either loss of a more distal region of 16p or rearrangement at the CBP locus, 16p13. (C) CBP protein expression is decreased in late passage HMEC-E6 cells. Early and late passage HMEC-LXSN vector controls (LXSN) (passages 11 and 16) and HMEC-E6 cells (E6) (passages 11 and 18) were analyzed for CBP protein expression as described in Materials and Methods. Equal amounts of protein lysate were loaded per lane. Actin was used as a loading control.
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