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Fig. 5. (A) Western blot analyses of HP1 ${alpha}$ , ß and ${gamma}$ proteins in A549 and HT29 cells treated with HDAC inhibitors. Protein levels for all treatments were normalized to the selected band (arrow) of total nuclear protein levels. (B) Interphase patterns of HP1 ${alpha}$ , ß (overlays) and ${gamma}$ (counterstain, immunostaining and overlay) studied by immunocytochemistry in A549 cells treated with TSA and NaBt. In order to demonstrate the location of HP1 ${gamma}$ in interchromatin compartments (white arrows) colour separations are also shown. Bars, 1 µm. (C) Nuclear radial distributions of HP1 foci ( ${alpha}$ , ß, ${gamma}$ ) in control (black line), TSA-treated cells (red line) and NaBt-treated cells (green line), showing the repositioning of HP1 proteins towards the nuclear interior. Average fluorescence intensities across the nucleus were determined using Andor iQ software.

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